CPY Document

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or a tr as ac tio n. A l p rim ar y so ur ce s ar e no t e qu al in v al ue . T yp ic al y, a ca de m ic h is to ria ns tu rn to s uc h p r i t e d r e c o r d s f o r th e st ar tin g po in t o f th ei r st ud y, M os t p le nt if ul a re g ov er ne nt a nd c hu rc h re co rd s, bu t n ew sp ap er s, d ia re s, p ri va te le tte rs , c or po ra te r ec or ds o f un iv er si tie s, or b us in es se s, li te ra ry te xt s, a nd m in ut es o f m ee tin gs a re o nl y a fe w o f t he so ur ce s th at c an b e g i t o u n c o v e r in fo nn at io n. O ff ci al r ec or ds o f th is s or t

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Characterization of the Hansenula polymorpha CPY gene encoding carboxypeptidase

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Characterization of the Hansenula polymorpha CPY gene encoding carboxypeptidase Y.

We have isolated the Hansenula polymorpha CPY gene encoding carboxypeptidase Y (Hp-CPY). The deduced amino acid sequence revealed that Hp-CPY consists of 541 amino acids and has a calculated Mr of 60,793. The protein is highly similar to Saccharomyces cerevisiae CPY (61.8% identity). At the N-terminus of Hp-CPY signals for the entry into the secretory pathway and subsequent sorting to the vacuo...

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ER-golgi traffic is a prerequisite for efficient ER degradation.

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Retention of a co-translational translocated mutant protein of carboxypeptidase Y of Saccharomyces cerevisiae in endoplasmic reticulum.

Co-translational translocation of Saccharomyces cerevisiae vacuolar glycoprotein carboxypeptidase Y (CpY) was highly efficient when studied with an in vivo and in vitro homologous system, comparison of limited proteolytic cleavage of immunoprecipitated translational products of CpY and subcellular localisation of a mutant CpY. The efficient segregation of CpY mRNA in highly purified fractions o...

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Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y

The structural gene for yeast vacuolar carboxypeptidase Y (PRC1) has been cloned by complementation of the prc1-1 mutation. As much as an eightfold elevation in the level of carboxypeptidase Y (CPY) results when a multiple-copy plasmid containing the PRC1 gene is introduced into yeast. Unlike the situation with a single copy of PRC1 in which newly synthesized CPY is efficiently localized to the...

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تاریخ انتشار 2005